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Exam 17: Aerobic Gram-Positive Cocci

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Question 1

Multiple Choice

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Yellow colonies on mannitol salt agar indicate the definitive presence of

A) methicillin-resistant Staphylococcus aureus.
B) Staphylococcus aureus.
C) Staphylococcus not S. aureus.
D) unable to definitively identify.

E) A) and B)
F) B) and C)

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D

Question 2

Multiple Choice

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The PBP2a latex agglutination test is used to detect catalase-positive, coagulase-positive, gram-positive cocci that are resistant to

A) vancomycin.
B) novobiocin.
C) oxacillin.
D) penicillin.

E) All of the above
F) A) and B)

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Question 3

Multiple Choice

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A catalase-positive, coagulase-negative, gram-positive coccus in clusters is isolated from the urine of a 24-year-old female. This isolate will require

A positive blood culture displays gram-positive cocci in chains. A sample is plated to blood, chocolate, and MacConkey agars. After 24 hours incubation in CO₂ (BA, CA) and ambient air (MAC) , all agar plates show no growth. An additional test was performed and the results are provided in the image provided. Based on these results, the organism is suspicious for Source: CDC and Dr. Mike Miller

Necrotizing fasciitis is caused by a

A translucent colony displaying subtle beta hemolysis is isolated from a placenta culture. The Gram stain revealed gram-positive cocci in pairs. The microbiologist drops 3% catalase reagent onto a colony on sheep blood agar, and it produces bubbles. He then performs a Staphyloccocus latex agglutination and the suspension remains smooth. The next step is to

Infections of indwelling devices, such as intravenous catheters, are often caused by

A blood culture yields yellow-pigmented, beta-hemolytic, catalase-positive, gram-positive cocci in clusters. The department is out of Staphylococcus latex agglutination reagent. The microbiologist sets up a tube coagulase and mannitol salt agar. She incubates both at 35°C in CO₂ overnight. The next day the coagulase test is negative and the mannitol salt agar displays yellow colonies. How will you proceed with these results?

The slide coagulase and the Staphylococcus latex agglutination tests are similar in that both test for the presence of

A leg wound isolate presents as a yellow pigmented, beta-hemolytic, catalase-positive, gram-positive cocci in clusters. Which of the following tests would you expect to be positive for this isolate?

The novobiocin resistance test is used to

The optochin disk test is incubated at/in _____________________.

The esculin/PYR disk test can be used to rapidly identify

An alpha-hemolytic, catalase-negative, gram-positive coccus that is susceptible to optochin can cause all of the following diseases except

The virulence factor most often associated with catalase-negative, gram-positive cocci that are bile soluble is

A catalase-negative coccus in pairs and short chains that is susceptible to ethyl hydrocupreine will also

A positive rapid esculin test appears

A patient, suspected to have bacterial endocarditis, had two sets of blood cultures drawn. All four bottles are positive with gram-positive cocci in pairs and chains. On sheep blood agar, the gray, transparent, alpha-hemolytic colonies are catalase negative, optochin resistant, ESC negative and PYR negative. Which of the following patient histories correlates with the presence of this organism?

A catalase-positive, gram-positive coccus that agglutinates in a slide coagulase test as well as in saline should be

A catalase-negative, gram-positive coccus in chains that is positive for esculin and PYR hydrolysis is also vancomycin resistant. What additional antibiotic resistance can this